The Dunn School Electron Microscopy (EM) Facility comprises state-of-the-art microscopes and offers the full gamut of EM techniques to facilitate a diverse range of biological and biomedical research. From scanning electron microscopy (SEM) and transmission electron microscopy (TEM), through to cutting-edge techniques such as correlative light and electron microscopy (CLEM), volume EM and elemental mapping using energy dispersive x-ray spectroscopy, we have the instrumentation and expertise to help you get the imaging results you need.
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JEOL 2100Plus 200kV TEM in use by Dr Yoel Klug, a postdoc in the Carvalho lab, to screen proteins associated with lipid droplet biogenesis
PhD student Wearn-Xin Yee (Tang lab) using the Zeiss Sigma 300 SEM to characterise the morphology of Neisseria gonorrhoeae mutants
The Facility is open to all members of the University, as well as to external researchers and industry. Our instrumentation includes:
We provide expert advice on which EM techniques are right for your project, as well as support in image interpretation and troubleshooting. We offer training on all of our instruments, as well as full service and collaboration options.
Usage charges are available upon request and vary depending on the instrument and project. Service work involves an additional fee. Quotes for grant applications can also be prepared.
The Facility is led by Dr Charlotte Melia and supported by Raman Dhaliwal. Both are expert electron microscopists and specialists in biological EM specimen preparation. Together, Charlotte and Raman collaborate on and facilitate a diverse range of research projects from across Oxford, spanning the areas of biophysics, structural biology, microbiology, cell biology, biomedicine, plant biology and more! Both enjoy the challenges associated with biological EM and are keen to help users at every step of the way in producing high quality images.
Please contact Charlotte for more information and to discuss how we can help you further your research. Enquires from industry are also welcome!
2023
Volume EM: a quiet revolution takes shape.
Collinson, L.M., Bosch, C., Bullen, A., Burden, J.J., Carzaniga, R., Cheng, C., Darrow, M.C., Fletcher, G., Johnson, E., Narayan, K., Peddie, C.J., Winn, M., Woods, C., Patwardham, A., Kleywegt, G. and Verkade, P.
Nature Methods – 20(6): 777-782.
2022
Density dependent regulation of inflammatory responses in macrophages.
Vaughan-Jackson, A., Stodolak, S., Ebrahimi, K.H., Johnson, E., Reardon, P.K., Dupont, M., Zhang, S., McCullagh, J.S. and James, W.S.
Front Immunol. – 13: 895488.
2020
Glutamine deprivation alters the origin and function of cancer cell exosomes.
Fan, S.J., Kroeger, B., Marie, P.P., Bridges, E.M., Mason, J.D., McCormick, K., Zois, C.E., Sheldon, H., Khalid Alham, N., Johnson, E. and Ellis, M.
EMBO J. – 39(16): e103009.
2019
Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury.
Davies, A.J., Kim, H.W., Gonzalez-Cano, R., Choi, J., Back, S.K., Roh, S.E., Johnson, E., Gabriac, M., Kim, M.S., Lee, J., Lee, J.E., et al.
Cell – 176(4): 716-728.e18.
2019
Membrane asymmetry imposes directionality on lipid droplet emergence from the ER.
Chorlay, A., Monticelli, L., Ferreira, J.V., M’barek, K.B., Ajjaji, D., Wang, S., Johnson, E., Beck, R., Omrane, M., Beller, M. and Carvalho, P.
Developmental Cell – 50(1): 25-42.e7.
2018
Drosophila PLP assembles pericentriolar clouds that promote centriole stability, cohesion and MT nucleation.
Roque, H., Saurya, S., Pratt, M.B., Johnson, E. and Raff, J.W.
PLoS Genetics – 14(2): e1007198.
2016
Neisseria cinerea isolates can adhere to human epithelial cells by type IV pilus-independent mechanisms.
Wörmann, M.E., Horien, C.L., Johnson, E., Liu, G., Aho, E., Tang, C.M. and Exley, R.M.
Microbiology – 162(3): 487-502.
2015
Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins.
Johnson, E., Seiradake, E., Jones, E. Y., Davis, I., Grünewald, K. and Kaufmann, R.
Science Reports – 5:9583.