Genome Engineering Oxford (GEO)

Genome Engineering Oxford (GEO)

Genome engineering allows precise, targeted changes to be made in the genomes of essentially any organism. It permits genetic analysis in systems previously unavailable to such manipulation, and greatly simplifies and speeds up the process in model organisms. The CRISPR system is a simple, adaptable and efficient method that can be used for this process.

The two-component CRISPR/Cas9 system of genome engineering.

Efficient mutagenesis of the AGO1 gene in a population in cultured cells.

Genome Engineering Oxford is a joint venture between the Dunn School of Pathology, DPAG, Biochemistry and Pharmacology. We provide a CRISPR design and testing service for generating experimentally tested vectors and are actively involved in developing new CRISPR methods and applications.

We offer advice on the optimal experimental strategy and vector choice for the system of interest, design sgRNAs for each target that minimise off-target effects, generate vectors, and test mutagenesis efficiency of each by high resolution melt analysis in a cell line from the organism of choice.

We can also provide experience, advice and assistance with the design and implementation of more involved projects involving genome engineering that are currently not routine and may involve development of novel methods. Current projects include: the design, manufacture and application of genome-wide and selective libraries to analyse cellular phenotypes; use of the technology in whole organisms; use of homologous targeting to generate, for example, point mutations, conditional floxed alleles, endogenous fluorescently tagged proteins; and, recruitment of chromatin modifying activities to alter epigenetic status and fluorescent proteins for live imaging of DNA sequences.

Projects are charged on a cost recovery basis, or by joint grant applications.

For more details, advice or information, please contact Joey Riepsaame (joey.riepsaame@path.ox.ac.uk, 01865 285489)

Joey Riepsaame
joey.riepsaame@path.ox.ac.uk