Flow Cytometry Facility

Flow Cytometry Facility

The Flow Cytometry Facility offers state-of-the-art service to scientists from the Dunn School as well as for the external users. We offer help and advice on panel design, flow data analysis and cell sorting.

Flow Cytometry is a powerful technique that allows you to test multiple parameters of your sample (i.e immunofluorescence analysis, cell-cycle and proliferation analysis, analysis of transfected fluorescent tags and many more) by fluorescence readout. Main advantages of this technique are accuracy and speed. You can analyse millions of cells in a matter of minutes and gather fluorescent data for every single cell you run through the analyser.

Facility users have access to 4 flow cytometric analysers, 3 cell sorters and an imaging cytometer - the Imagestream (please check equipment for details). After training users gain 24/7 access to analysers and can operate them on their own. Cell sorting is offered as a service, cell sorters are operated by dedicated staff only. Cell sorting service - this powerful technique allows you to enrich a population of interest or source single cells of interest from cell suspension. Cells after this procedure are viable, resuspended in media of your choice. Cells can be placed in different types of collection tubes or plates (24wp, 96wp, 384wp).

Please contact us if you like to sign up for training and with any inquiries. 

 

Flow Cytometers (analysers)

Analysers are available for users 24/7. Please contact for details.

BD Calibur – has 2 lasers 488 and 640 nm. It has 4 fluorescent channels available. Cytometer is equipped with HTS (High Throughput System – 96wp reader).

Cytek DxP8 – has 3 lasers 405, 488 and 640 nm. It has 8 fluorescent channels available

CyAn ADP – has 3 lasers 405, 488 and 640 nm. It has 9 fluorescent channels available

BD Fortessa X20 - has 5 lasers: 355 (UV), 405, 488, 561 and 640 nm. It has 18 fluorescent channels available. Cytometer is equipped with HTS (High Throughput System – 96wp reader).

 

Cell Sorters

Sorts are performed by authorised operators only.

Flow lab is equipped with two jet-in-air hi-speed sorters: MoFlo Astrios and MoFlo XDP (both from Beckman Coulter) and one cuvette cell sorter (Aria III from BD). Sorters are operated by authorised personnel only.

Astrios is equipped with 4 lasers: 405, 488, 561 and 640 nm and is able to detect up to 14 fluorochromes. This sorter is in a 2 class hood.

MoFlo XDP has 3 lasers: 355(UV), 488 and 640 nm and has 8 fluorescent channels.

BD FACSAria III is equipped with 4 lasers: 405, 488, 561 and 635 nm and has 16 fluorescent channels.

We offer high speed bulk sorts (up to 30 000 events per second), and plate sorts (single cell deposition) with index sort option.

 

Imaging Flow Cytometer

Amnis Imagestream MKII imaging flow cytometer – cutting edge technology available for lab users. Cytometer is equipped with 4 lasers: 405, 488, 561 and 638 nm offering 12 fluorescent channels. Technology behind the machine allows to take a high resolution picture (optical magnification of 20x, 40x or 60x) of every single event and overlay flow data with microscopy picture.

 

Michal Maj
michal.maj@path.ox.ac.uk