Flow Cytometry Facility

Flow Cytometry Facility

The flow cytometry facility offers service for scientists from Dunn School as well as for external users. Every user can find help and advice on panel design, flow data analysis and cell sorting. We offer an individual instrument training as well as assistance in data acquisition.

Cell sorting:
 

Flow lab is equipped in two jet-in-air hi-speed sorters: Astrios and MoFlo XDP (both from Beckman Coulter). Sorters are operated by authorised operator only.

Astrios is equipped with 4 lasers: 405, 488, 561 and 640 nm and is able to detect up to 14 fluorochromes. This sorter is in a 2 class hood.

MoFlo XDP has 3 lasers: 355(UV), 488 and 640 nm and has 8 fluorescent channels.

We offer high speed bulk sorts (up to 30 000 events per second), and plate sorts (single cell deposition) with index sort option.

Flow Analysis:
 

We have 4 analysers in our lab:

BD Calibur – has 2 lasers 488 and 640 nm, is able to detect up to 4 fluorochromes. Cytometer is equipped with HTS (High Throughput System – 96wp reader).

Cytek DxP8 – has 3 lasers 405, 488 and 640 nm is able to detect up to 8 fluorochromes.

CyAn ADP – has 3 lasers 405, 488 and 640 nm and is able to detect up to 9 different fluorochromes.

Amnis Imagestream MKII imaging flow cytometer – cutting edge technology available for lab users. Cytometer is equipped with 4 lasers: 405, 488, 561 and 638 nm offering 12 fluorescent channels. Technology behind the machine allows to take a high resolution picture (optical magnification of 20x, 40x or 60x) of every single event and overlay flow data with microscopy picture.

Read more about instruments configuration

Data analysis:
 

We offer help, training and licenses for FlowJo, Kaluza and Ideas analysis software.

Please contact Michal Maj (michal.maj@path.ox.ac.uk tel: 01865 285 479) with any inquiries.

Michal Maj
michal.maj@path.ox.ac.uk