Simon Hunt
University Lecturer in Immunology, Departmental academic librarian
The Hunt Laboratory
Cell population array imaging
| simon.hunt@path.ox.ac.uk | |
| Contact address | Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE |
| Department | Sir William Dunn School of Pathology |
Simon Hunt
Our laboratory’s chief effort is to develop a new instrument for surveying the fluorescence of thousands of individual single cells over time, the “cell population array imager”. This low-cost, portable device can measure, for example, calcium transients in a large number of lymphocytes after membrane-receptor cross-linking. Employing automated classification algorithms based on neural networks, we study whether cells can usefully be grouped into subsets by their biochemical signalling function.
Research Details
- Development of the cell population array imager: “CPAI”. Visit www.cpai.eu. Systems studied or being considered include:
- T cell signalling
- B cell signalling
- Intracellular enzyme assays using fluorogenic substrates such as the enzymes used therapeutically in lysosomal storage disease
- Measurement of intracellular calcium signals during lymphocyte, neutrophil and erythrocyte stimulation
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Analysis of calcium entry pathways into lymphocytes and erythrocytes by perturbation with dihydropyridines and other channel-specific drugs
